✦ LIBER ✦

Cationic heme undecapeptide as stain for detecting glycosaminoglycans on cellulose acetate strips

✍ Scribed by Nicolae Ghinea

Book ID: 102986210
Publisher: Elsevier Science
Year: 1986
Tongue: English
Weight: 864 KB
Volume: 155
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(86)90227-7

No coin nor oath required. For personal study only.

✦ Synopsis

A rapid and sensitive method has been developed for detecting glycosaminoglycans on cellulose acetate strips. The method is based on the electrostatic interactions between glycosaminoglycans and cationic heme undecapeptide. The complexes are identified by peroxidatic reaction using 3,3'-diaminobenzidine as H2 donor. The detection limit is 2.5-10 ng for sulfated glycosaminoglycans and 60 ng for hyaluronic acid. The sensitivity of this method is higher than of those using Alcian blue or toluidine blue (500 ng).