## Abstract A capillary zone electrophoresis (CZE) method with preceding cationic transient capillary isotachophoresis (tCITPโCZE) was developed for uncoated fusedโsilica capillaries to analyze metalโbinding proteins (MBPs) of clinical relevance. UV detection was followed by mass spectrometry (MS).
Cationic capillary isotachophoresis of protein
โ Scribed by Frederick S. Stover
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 392 KB
- Volume
- 445
- Category
- Article
- ISSN
- 1873-3778
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## Performance of narrow range ampholytes and a discrete spacer mixture is evaluated for improved protein separations by cationic isotachophoresis. A spacer mixture containing 22 cations is developed and relative step heights of components are presented. Different ampholytes and the discrete spac
Isotachophoresis in polyacrylamide gel tubes (PAG-ITP) and in capillary tubes (Tachophor, LKB) have previously been found by the authors, to be very promising high-separation methods for CSF and serum proteins, especially regarding the diagnosis of MS. PAG-ITP methods for analytical and preparative