Weak anion and cation exchange mixed-bed
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Chunyan Hou; Huiming Yuan; Xiaoqiang Qiao; Jinxiang Liu; Yichu Shan; Lihua Zhang
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Article
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2010
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John Wiley and Sons
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English
โ 191 KB
## Abstract To separate proteins with a wide distribution of p__I__s under the conditions compatible to online tryptic digestion (with preferable pH=8.0), weak anion and cation exchange chromatography (WAX/WCX) mixedโbed microcolumn has been developed. With a mixture of five proteins with p__I__s r