Cation-independent mannose 6-phosphate receptor contains covalently bound fatty acid

✍ Scribed by Keith R. Westcott; Leonard H. Rome

• Book ID: 102876180
• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 726 KB
• Volume: 38
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.240380104

✦ Synopsis

The cation-independent mannose 6-phosphate receptor ( 2 15 ,OOO daltons) was isolated from embryonic bovine tracheal cells and embryonic human skin fibroblasts labelled with ['H]palmitic acid. the tritium label was detected in the protein upon fluorographic analysis of SDS-polyacrylamide gels of the purified receptor. The label was not sensitive to hydroxylamine, methanolic KOH, or P-mercaptoethanol, but labelled fatty acid was recovered from the protein by acidic methanolysis. Labelled receptor protein could not be isolated from cells grown in the presence of [3H]myristic acid. The results suggest the presence of amide-linked palmitic acid in the structure of the cation-independent mannose 6-phosphate receptor.