A radiochemical microassay for soluble and membrane-bound glutamate decarboxylase (GAD) is described. Up to 180 samples can be determined per day with a variation coefficient of 2%. The method detects newly synthesized gamma-amino-n-butyric acid in the picomole range and can easily be applied to oth
Cation binding to biomolecules. the screening of the electrostatic potential of the phosphate group by mono- and divalent cations.
โ Scribed by A. Pullman; H. Berthod
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 409 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0009-2614
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โฆ Synopsis
It is shown that the effect of the binding of an alkali and alkaline-earth cation on the properties of a molecule may be evaluated by replacing the cation by an appropriate pcint charge. The procedure is utilized to evaluate the effect of a monoor divalent ion placed in different positions on the molecuiar potential of the phosphodiester linkage. The resulting specific screening of the phosphate potential is discussed.
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A spiro orthoester having an ester moiety, 2-acetoxymethyl-1,4,6trioxaspiro[4.6]undecane (4) was synthesized, and its cationic polymerization and depolymerization of the obtained polymer (5) were carried out. The monomer 4 underwent cationic polymerization with a cationic catalyst to afford the corr