Cation binding effects on the pH, thermal and urea denaturation transitions in α-lactalbumin

Effects of urea-thermal denaturation on

Effects of urea-thermal denaturation on the high-performance cation-exchange chromatography of α-chymotrypsinogen-A

✍ Ernest S. Parente; Donald B. Wetlaufer 📂 Article 📅 1984 🏛 Elsevier Science 🌐 English ⚖ 707 KB

Retention parameters of a-chymotrypsinogen-A were determined by isocratic elution for a series of concentrations of calcium acetate and sodium acetate both in the presence and absence of urea. Under non-denaturing conditions of temperature and urea concentration, urea facilitated elution. Under reve