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Catechol-O-methyltransferase activity in CHO cells expressing norepinephrine transporter

✍ Scribed by Elodie Percy; David M Kaye; Gavin W Lambert; Sara Gruskin; Murray D Esler; Xiao-Jun Du


Book ID
110004570
Publisher
Nature Publishing Group
Year
1999
Tongue
English
Weight
212 KB
Volume
128
Category
Article
ISSN
0007-1188

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Catechol-O-methyltransferase activity in
✍ Ilkka ReenilΓ€; PΓ€ivi Tuomainen; Seppo Soinila; Raimo K. Tuominen; Pekka T. MΓ€nni πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 924 KB

To analyze the intracellular catechol-O-methyltransferase (COMT) activity in cultured cells, a substrate (dihydroxybenzoic acid) was added to the tissue culture plates and the product (vanillic acid) was analyzed with reversed-phase HPLC with coulometric detection. The cultures, prepared from variou