Catalytic resonance scattering spectral determination of ultratrace horseradish peroxidase using rhodamine S

✍ Scribed by Zhiliang Jiang; Yueyuan Liang; Guoxia Huang; Xiaoling Wei; Aihui Liang; Fuxin Zhong

• Book ID: 101714216
• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 225 KB
• Volume: 24
• Category: Article
• ISSN: 1522-7235
• DOI: 10.1002/bio.1079

✦ Synopsis

Abstract

A highly sensitive and selective resonance scattering spectral assay was proposed for the determination of horseradish peroxidase (HRP), based on its catalytic effect on the H~2~O~2~ oxidation of KI to form I~3~^−^. The I~3~^−^ combined respectively with rhodamine (Rh) dye such as rhodamine S (RhS), rhodamine 6G (Rh6G), rhodamine B (RhB) and butyl‐rhodamine B (b‐RhB), to form association particles (Rh‐I~3~)~n~. The four Rh systems all exhibit a stronger resonance scattering (RS) peak at 424 nm. For the RhS, Rh6G, RhB and b‐RhB systems, HRP concentration in the range of 3.2 × 10^−12^ to 4.8 × 10^−9^, 2 × 10^−11^ to 3.2 × 10^−9^, 1.6 × 10^−11^ to 3.2 × 10^−9^ and 1.6 × 10^−11^ to 4 × 10^−9^g/mL was linear to its RS intensity at 424 nm, with a detection limit of 2.2 × 10^−12^, 2.5 × 10^−12^, 4.4 × 10^−12^ and 2.6 × 10^−12^g/mL, respectively. This RhS system was most sensitive and stable, and was applied for the determination of HRP in the hepatitis B surface antibody labeling HRP and water samples, with satisfactory results. Copyright © 2009 John Wiley & Sons, Ltd.