Catalytic Properties of Carbonyl Reductase from Rabbit Kidney for Acetohexamide and Its Analogs
✍ Scribed by Y. Imamura; T. Higuchi; M. Otagiri; S. Nagumo; H. Akita
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 410 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0045-2068
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✦ Synopsis
Analogs submitted by ethyl, (n)-propyl, (n)-butyl, and isopropyl groups instead of methyl group adjacent to a ketone group of acetohexamide were synthesized and the structural requirements of carbonyl reductase from rabbit kidney for these analogs were kinetically examined. The hydrophobicities in straight-chain alkyl groups of acetohexamide analogs were found to play an important role in the catalytic activity and substrate-binding capacity of the enzyme. We propose the possibility that a hydrophobic pocket is located in the substrate-binding domain of the enzyme. 1994 Academic Press, Inc.