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Catalytic durability of magnetoproteoliposomes captured by high-gradient magnetic forces in a miniature fixed-bed reactor

✍ Scribed by Marcel De Cuyper; Bruno De Meulenaer; Pol Van der Meeren; Jan Vanderdeelen


Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
541 KB
Volume
49
Category
Article
ISSN
0006-3592

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✦ Synopsis


Cytochrome c-oxidase, used as a model membranebound enzyme, was embedded in phospholipid bilayer membranes, attached to nanometer-sized Fe,O, colloids (so-called magnetoliposomes). In comparison with the lipid-depleted free enzyme, both the activity and the enzymatic stability of the complexes, stored at 4"C, were considerably enhanced. These beneficial properties of magnetoproteoliposomes have been successfully exploited in a magnetically controlled fixed-bed bioreactor, operating in a continuous flow regime. 0 1996