Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions

Use of Surface Plasmon Resonance for Rea

Use of Surface Plasmon Resonance for Real-Time Measurements of the Global Conformational Transition in Human Phenylalanine Hydroxylase in Response to Substrate Binding and Catalytic Activation

✍ Torgeir Flatmark; Anne Jorunn Stokka; Sissel V. Berge 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 93 KB

In the present study the optical biosensor technique, based on the surface plasmon resonance (SPR) phenomenon, was used for real-time measurements of the reversible binding of the pterin cofactor (6R)-l-erythro-5,6,7,8-tetrahydrobiopterin (BH(4)) and l-phenylalanine (l-Phe) to human phenylalanine hy