A new method for labeling antibodies which involves selenol-catalyzed reduction of native disulfide bonds in antibodies to generate thiol groups, which then are labeled using thiol-reactive reagents, is described. The reduction and labeling steps of this rapid procedure are carried out in one vessel
β¦ LIBER β¦
Catalysis of Reduction of Disulfide by Selenol
β Scribed by Rajeeva Singh; Lana Kats
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 127 KB
- Volume
- 232
- Category
- Article
- ISSN
- 0003-2697
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The reactions of the title compounds are governed by sulfur redox chemistry. Thus, cyanothioformamide 2 reacts with aryl isocyanates 3a-c to give bis[3-aryl-1-(arylcarbamoyl)-2oxo-4-(arylcarbamoylimino)imidazolidin-5-yl] disulfides 6, as shown by an X-ray crystallographic investigation of product 6a