Catalysis of Amide Proton Exchange by the Molecular Chaperones GroEL and SecB
β Scribed by Zahn, R.; Perrett, S.; Stenberg, G.; Fersht, A. R.
- Book ID
- 121155729
- Publisher
- American Association for the Advancement of Science
- Year
- 1996
- Tongue
- English
- Weight
- 548 KB
- Volume
- 271
- Category
- Article
- ISSN
- 0036-8075
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The rates of hydrogen exchange were measured in a ''physiological'' denatured state of staphylococcal nuclease using a NMR magnetization transfer experiment suitable for the measurement of exchange rates faster than 0.5 s 21 . The results are compared with predicted exchange rates (k ex ) for the ra
## Abstract Backbone amide proton exchange rates in the DNAβbinding domain of GAL4 have been determined using ^1^Hβ^15^N heteronuclear correlation NMR spectroscopy. Three forms of the protein were studied β the native Znβcontaining protein, the Cdβsubstituted protein, and a ZnβGAL4/DNA complex. Exc