## Abstract Exoglucanase and endoglucanase (glucanases) enzymes of __S. albaduncus__ were found to be very stable, showing only 36% and 8% loss in activities respectively after 3 days of incubation at 50 ยฐC. In contrast, ฮฒโglucosidase was significantly less stable retaining only 22.30% activity aft
Catabolite repression of cellulase biosynthesis in Streptomyces albaduncus
โ Scribed by R. K. Harchand; Dr. S. Singh
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 481 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0233-111X
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โฆ Synopsis
The cellulase complex of S. albaduncus was found to undergo catabolite repression in the presence of glycerol accompanied by a decrease in the pH of the medium. The results of the experiments conducted at two different initial pH of the medium suggested that pH effect as well as proteases were involved in the inactivation of glucanases (exoglucanase and endoglucanase) whereas b-glucosidase inactivation was solely due to 'pH effect'. It was also apparent that pH change followed by the addition of glycerol caused alterations in protease activities as well as pattern of their production.
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We have isolated and characterized a pleiotropic recessive mutation,fen2-l, that causes resistance to fenpropimorph and a low level of ergosterol in Saccharomyces cerevisiae. Ergosterol synthesis in the mutant strain was 5.5-fold slower than in the wild type; however, in vitro assays of the enzymes
## Abstract Modeling the role of cyclic AMP (cAMP) in catabolite repression of inducible enzyme production in microbial cells was studied. A catabolite repression index, __F__, was defined based on the postulation that complex formation occurs between RNA polymerase (RNAP) and DNA, and shifting fro