Catabolism of caffeine and related purine alkaloids in leaves ofCoffea arabicaL.
β Scribed by H. Ashihara; A. M. Monteiro; T. Moritz; F. M. Gillies; A. Crozier
- Publisher
- Springer-Verlag
- Year
- 1996
- Tongue
- English
- Weight
- 605 KB
- Volume
- 198
- Category
- Article
- ISSN
- 0032-0935
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In the leaves of Coffea arabica L., purine alkaloid formation was estimated by analyzing the theobromine and caffeine content and by measuring the methylation rate of [2-1~C]theobromine to [2-14C]caffeine in short-term experiments (6-24 h). At the same time, growth (in terms of dry weight and area),
## Abstract Previously isolated strain of __Pseudomonas__ sp. has the capability of utilizing caffeine as the sole source of carbon and nitrogen and degrading caffeine at higher concentrations (>10 g l^β1^). In this study, an assay has been developed to study the enzymatic conversion of caffeine t