Cover
Title Page
Copyright Page
Dedication
Contents
Biography
Preface
Chapter 1 --
Introduction to Scientific Method from a Cellular Perspective
Subchapter 1.1 --
Scientific Method in Action [1]
Introduction
Background
Methods
Cell cultures
Microinjection
Electron microscopy
Calculation of nuclear uptake and size distribution
Results
Reference
Chapter 2 --
Cellular Biodiversity
Subchapter 2.1 --
Cellular Biodiversity on the High Seas [1]
Introduction
Background
Methods
Cell culture and population density
Characterization of photosynthetic pigments
Results. Subchapter 2.2 --
The Mystery of the Missing Mitochondria [2]Introduction
Background
Methods
Subcellular fractionation of giardia
Transmission electron immunomicroscopy
Results
References
Chapter 3 --
Proteins
Subchapter 3.1 --
Bite of the Brown Recluse Spider: An Introduction to Protein Gel Electrophoresis [1]
Introduction
Background
Methods
Red blood cell ghosts
Venom digestion assays
Protein gel electrophoresis
Results
Subchapter 3.2 --
Where to Start? A Case of Too Many AUGs [3]
Introduction
Background
Methods
Yeast culture conditions. CDC9-GFP fusions, CDC9 mutagenesis, and HA taggingSubcellular fractionation and protease K digestion
Immunoblotting
Results
Subchapter 3.3 --
Cotranslational Translocation: Gatekeepers of the RER [4]
Introduction
Background
Methods
In vitro translation
Crosslinking assay
Flotation assay
Protease resistance assay
Results
Subchapter 3.4 --
From Sequence to Function [5]
Introduction
Background
Methods
Isolation and structural analysis of bovine AQP1
Data processing
Results
Subchapter 3.5 --
Visualizing Protein Conformation [6]
Introduction
Background
Methods. Protein expression and purificationElectron microscopy
SNARE complex labeling
Results
Subchapter 3.6 --
Second Chance Chaperones: How Misfolded Proteins get Refolded [7]
Introduction
Background
Methods
Purification of Hsp104
Purification of Hsp40 and Hsp70
Preparation of yeast lysates
Turbidity assay
Refolding assays
Gel filtration
Results
References
Chapter 4 --
The Nucleus
Subchapter 4.1 --
Nuclear Pore Complex Assembly is a House of Cards [1]
Introduction
Background
Methods
Nuclear reconstitution assay
Immunodepletion
Nuclear import
Immunoblotting
DNA replication assay. Scanning electron microscopyResults
Subchapter 4.2 --
Importin b: The Important Importin [2]
Introduction
Background
Methods
Preparation of a high-speed supernatant from Xenopus eggs
Immunodepletion of importin from cytosol
Nuclear protein import assay
Nuclear localization signal binding assay
Protein gel electrophoresis and immunoblotting
Results
Subchapter 4.3 --
A Tale of tRNA Transport [3]
Introduction
Background
Methods
In vitro translation and 35S-labeling
Injection into Xenopus frog eggs
In vitro binding assays
Gel electrophoresis and immunoblotting
Results. Subchapter 4.4 --
When It Comes to the Nucleus --
Size Matters [4]