Cascade impactor method for the droplet size characterization of a Metered-dose nasal spray
✍ Scribed by Cheng Der Yu; Richard E. Jones; Maida Henesian
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 685 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0022-3549
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✦ Synopsis
both assays (Table 11). The accuracy of the methods was further validated by comparing results from the assay of captopril and its disulfide metabolites by the GC-EC method with results from the GC-MS assay (15) in plasma from dogs that had been given oral doses of captopril (2.5 mghg). The generally excellent agreement obtained for the two methods is shown in Table 111.
The GC-EC assay described appears suitable for determining blood concentrations of unchanged captopril and plasma concentrations of captopril and its disdfide metabolites in samples from dogs and humans. In the range of blood concentrations (captopril) from 20 to 200 ng/mL and plasma concentrations (captopril and its disulfide metabolites) from 50 to lo00 ng/mL, the linearity, reproducibility, precision, and accuracy of the assays were generally excellent. The lower limits for quantitation of captopril and its disulfide metabolites by the GC-EC method are comparable to the existing GC-MS (15) and liquid chromatographyelectrochemical detector methods (16, 17). The thin-layer radiochromatographic method ( 18) is more sensitive when high specific activity [14C]captopril is used. The GC-EC method is 4to 10-fold more sensitive than the other quantitative methods (12, 14) previously reported.