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Cardiac sarcoplasmic reticulum isolation from slaughterhouse beef heart

✍ Scribed by George W. Lighty Jr.; Helen A. Bertrand

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
847 KB
Volume
99
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method by which slaughterhouse beef heart may be used to obtain a cardiac sarcoplasmic reticulum with high sarcoplasmic reticulum activities and low non-sarcoplasmic reticulum contamination is described. Moreover conditions which influence the yield and activity of this preparation are extensively explored. The characteristics of this sarcoplasmic reticulum preparation are compared with the preparations obtained by the other available methods.

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In order to study the conductances of the Sarcoplasmic Reticulum (SR) membrane, microsomal fractions from cardiac SR were isolated by differential and sucrose gradient centrifugations and fused into planar lipid bilayers (PLB) made of phospholipids. Using either KCl or K-gluconate solutions, a large