Carcinoma of type II pneumocytes. PAS staining as a screening test for nuclear inclusions of surfactant specific apoprotein
✍ Scribed by Gurmukh Singh; Sikandar L. Katyal; Chikao Torikata
- Book ID
- 102666622
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 963 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
Bronchioloalveolar carcinomas (n = 15) and other primary lung carcinomas (n = 9) were studied by PAS staining. The bronchioloalveolar carcinoma group contained three cases of carcinomas of type I1 pneumocytes. Cytoplasmic staining with PAS was most intense in mucus secreting carcinomas. Carcinomas of type 11 pneumocytes could not be differentiated from other tumors on the basis of cytoplasmic PAS staining. However, PAS positive nuclear "inclusion" were seen only in the three carcinomas of type I1 pneumocytes. The study indicates that PAS staining, though nonspecific, may serve as a screening test for carcinomas of type I1 pneumocytes by highlighting the nuclear inclusions of surfactant specific apoprotein.
Cancer 50:946-948, 1982.
ULMONARY SURFACTANT, predominantly a lipid, P has been shown to be a lipoprotein.' ' The surfactant specific apoprotein in rats and primates has been shown to be, mostly, a 38,000 dalton molecular weight glycoprotein which exhibits staining with periodic acid Schiff reagent (PAS) in acrylamide gels.4 We have prepared antisera specific for rat and primate surfactant apoprotein.'.' The antisera specifically stain type I1 pneurn~cytes.~.'*~ It has been shown that the heterogeneous group of bronchioloalveolar carcinomas contains a subtype of tumors arising from type I1 pneumocytes.'~'
We earlier reported the findings from a study using irnmunoperoxidase staining for surfactant apoprotein in "bronchioloalveolar" carcinoma^.^ The cytoplasm of tumor cells in three cases stained with rabbit antimonkey surfactant specific antiserum indicating that the tumors were, in all likelihood, carcinomas of type I1