𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Carbonic anhydrase II is an AP-1 target gene in osteoclasts

✍ Scribed by J.-P. David; M. Rincon; L. Neff; W.C. Horne; R. Baron

Publisher
John Wiley and Sons
Year
2001
Tongue
English
Weight
209 KB
Volume
188
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

c‐Fos, a member of the AP‐1 family of transcription factors, is necessary for osteoclast differentiation but to date, none of the osteoclast‐phenotypic markers have been identified as AP‐1 target genes. Here, we demonstrate that carbonic anhydrase II (CA II), an enzyme necessary for osteoclast activity, is transcriptionally upregulated by c‐Fos/AP‐1. A functional AP‐1 binding site is present in the CA II promoter and is necessary for this regulation. Furthermore, we show that AP‐1 binding activity, mainly composed of Fra‐2 and JunD, is induced by treatment of bone marrow cultures with the osteoclastogenic hormone 1,25 dihydroxyvitamin D~3~. Fra‐2 and JunD are found in mature osteoclasts as well. Thus, our data demonstrate that cFos/AP‐1 can directly regulate the expression of this osteoclast marker and that AP‐1 activity is upregulated in osteoclast progenitors in response to osteoclastogenic signals. © 2001 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Successful DNA-based prenatal diagnosis
Successful DNA-based prenatal diagnosis of the 728+1 g→t mutation at the exon 6-intron 6 junction in the carbonic anhydrase II gene
✍ M. Rafiq Islam; Pietro Strisciuglio; Giuseppe Bonapace; William S. Sly 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 146 KB 👁 1 views

and three siblings (aged 17, 20 and 22 years) also had an elongated chromosome 16 short arm. During counselling, the parents were informed of the familial nature of the anomaly. They were informed that the cytogenetic characterization of the chromosome 16 anomaly and the information derived from pre