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Carbonic anhydrase adsorption in porous silicon studied with infrared ellipsometry

✍ Scribed by Arwin, H. ;Karlsson, L. M. ;Kozarcanin, A. ;Thompson, D. W. ;Tiwald, T. ;Woollam, J. A.


Book ID
105363181
Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
150 KB
Volume
202
Category
Article
ISSN
0031-8965

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✦ Synopsis


Abstract

The refractive index N = n + ik of the protein bovine carbonic anhydrase (BCA) adsorbed in porous silicon layers is determined using infrared spectroscopic ellipsometry. Five absorption bands in the amide region are resolved. The distribution of the protein inside the porous matrix is modelled and it is found that more BCA per internal surface area are adsorbed in the upper part of the porous layer than in the bottom part. (Β© 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)


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