Carbon-13 NMR study of the B9(Asp) mutant of human insulin: Sequence-specific carbon-13 assignments and structural information

✍ Scribed by Søren M. Kristensen; Jens J. Led

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 851 KB
• Volume: 33
• Category: Article
• ISSN: 0749-1581
• DOI: 10.1002/mrc.1260330610

✦ Synopsis

Abstract

The resonances of the proton‐bearing ^13^C nuclei at natural abundance were assigned for the solution dimer of the B9(Asp) mutant of human insulin. The assignments are based on a combination of direct and relayed ^1^H^13^C correlated spectra, i.e. SQC, SQC‐HOHAHA and HMQC‐COSY, and the previously assigned ^1^H spectrum. The study proves valuable not only because of the ^13^C assignments, but also because it provides a verification, a completion and a few corrections of the previous ^1^H assignments. Finally, the obtained ^13^C assignments support the previously reported correlation between the secondary ^13^C~α~ and ^13^C~β~ chemical shifts and the secondary structure of proteins.