Exonuclease III (Exo III) of Escherichia coli is a DNA-modifying enzyme very frequently used in molecular biology. The experiments described here were carried out with the aims of reliably controlling exonuclease activity and of learning more about the enzyme's specificities. The dependence of Exo I
Carboligatic activity of escherichia coli
โ Scribed by Reynolds, H. ;Werkman, C. H.
- Publisher
- Springer-Verlag
- Year
- 1937
- Weight
- 241 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0003-9276
No coin nor oath required. For personal study only.
โฆ Synopsis
The formation of acetylmethylcarbinol from glucose by bacteria of the genus Aerobacter has proved to be of distinct value in differentiating the genus from Escherichia. The reliability of the Voges-Proskauer test in differentiating the colon and aeroge~es sections has never been questioned and it is of interest in connection with the work to be reported that organisms of the cell section which are regularly Voges-Proslcauer negative are shown to possess the carboligatic mechanism which is responsible for the formation of acetylmethylcarbinol. However, under the usual conditions of culturing, the carboligase system does not function.
๐ SIMILAR VOLUMES
Highly active salt-washed ribosomes from Escherichia coli are isolated by gel filtration on Sephacryl S-200 in a buffer containing 1 M ammonium chloride and putrescine, spermidine, calcium, and magnesium ions. Up to several hundred grams of cells can be processed in less than 24 h. The ribosome solu