Using flow cytometry, spherulating nuclei of Physarum isolated at the beginning of spherule wall formation were found to exhibit a DNA content corresponding to the G phase of the cell cycle, although 8% lower. Before the first mitosis after spherule germination, a very slight incorporation of H thym
Carbohydrate metabolism during differentiation (Sclerotization) of the myxomycete Physarum flavicomum
โ Scribed by Lynch, Thomas J. ;Henney, Henry R.
- Publisher
- Springer-Verlag
- Year
- 1973
- Weight
- 516 KB
- Volume
- 90
- Category
- Article
- ISSN
- 0003-9276
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โฆ Synopsis
The metabolism os carbohydrates during differentiation (sclerotization) of Physarum/lavicomum was studied using the radiorespirometric technique.
After about 36 h in a selerotization (starvation) medium the metabolism declined to a level characteristic of the dormant state. Sclerotia incubated in complete growth medium quickly reverted to a metabolically active state and by 9 h they regained about 50~ of their metabolic potential.
Sclerotia metabolize carbohydrates by the Embden-Meyerhof-Parnas (EMP)tricarboxylie acid and the pentose phosphate (PP) pathways. Compared to growing plasmodia the activity os the E~P is reduced to a greater extent than the PP in sclerotia. Also, EMP-produced triose phosphates are not well equilibrated: ~here is a greater yield os itCO~ from the C-4 of glucose than from the C-3; the C-3 is incorporated into the lipid fraction to a greater extent than the C-4.
The metabolism of carbohydrates by sclerotia is stimulated by eyclic-3'-5'adenosine monophosphate.
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