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Carbohydrate metabolism during differentiation (Sclerotization) of the myxomycete Physarum flavicomum

โœ Scribed by Lynch, Thomas J. ;Henney, Henry R.


Publisher
Springer-Verlag
Year
1973
Weight
516 KB
Volume
90
Category
Article
ISSN
0003-9276

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โœฆ Synopsis


The metabolism os carbohydrates during differentiation (sclerotization) of Physarum/lavicomum was studied using the radiorespirometric technique.

After about 36 h in a selerotization (starvation) medium the metabolism declined to a level characteristic of the dormant state. Sclerotia incubated in complete growth medium quickly reverted to a metabolically active state and by 9 h they regained about 50~ of their metabolic potential.

Sclerotia metabolize carbohydrates by the Embden-Meyerhof-Parnas (EMP)tricarboxylie acid and the pentose phosphate (PP) pathways. Compared to growing plasmodia the activity os the E~P is reduced to a greater extent than the PP in sclerotia. Also, EMP-produced triose phosphates are not well equilibrated: ~here is a greater yield os itCO~ from the C-4 of glucose than from the C-3; the C-3 is incorporated into the lipid fraction to a greater extent than the C-4.

The metabolism of carbohydrates by sclerotia is stimulated by eyclic-3'-5'adenosine monophosphate.


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