Carbamyl phosphate synthesis inBacillus
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Barry Potvin; Harry Gooder
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Article
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1975
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Springer
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English
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In vitro and "in situ" assays have been developed to test the carbamyl phosphate synthetase (CPSase) activity of a series of pyrimidine-requiring mutants of Bacillus subtilis. The enzyme has been shown to be highly unstable, and was successfully extracted only in the presence of 10% glycerol and 1 m