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Carbamyl phosphate biosynthesis in Bacillus subtilis

โœ Scribed by Inda M. Issaly; Abel S. Issaly; Jose L. Reissig


Book ID
115732283
Publisher
Elsevier Science
Year
1970
Weight
760 KB
Volume
198
Category
Article
ISSN
0005-2744

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Carbamyl phosphate synthesis inBacillus
โœ Barry Potvin; Harry Gooder ๐Ÿ“‚ Article ๐Ÿ“… 1975 ๐Ÿ› Springer ๐ŸŒ English โš– 1005 KB

In vitro and "in situ" assays have been developed to test the carbamyl phosphate synthetase (CPSase) activity of a series of pyrimidine-requiring mutants of Bacillus subtilis. The enzyme has been shown to be highly unstable, and was successfully extracted only in the presence of 10% glycerol and 1 m