Capillary zone electrophoretic separations of proteins in polyethylene glycol-modified capillaries

✍ Scribed by G.J.M. Bruin; J.P. Chang; R.H. Kuhlman; K. Zegers; J.C. Kraak; H. Poppe

• Book ID: 104144562
• Publisher: Elsevier Science
• Year: 1989
• Tongue: English
• Weight: 506 KB
• Volume: 471
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)94190-6

✦ Synopsis

Fused-silica capillaries were wall modified with y-glycidoxypropyltrimethoxysilane and polyethylene glycol600 in order to decrease the influence of wall adsorption in capillary zone electrophoretic separations of proteins. It is shown that a significant decrease in adsorption is obtained and electro-osmotic flow is also diminished. For the proteins studied, symmetrical peaks were obtained in the pH range 3-5. However, some adsorption still occurs as the plate numbers are below theoretical expectations. At higher pH values appreciable peak deformations and drastic decreases in resolving power are obseryed. The procedure allows the rapid and efficient separation of protein mixtures suitable for separation in the indicated pH range, and the coating shows a good stability.