Capillary zone electrophoresis of biological substances with surface-modified fused silica capillaries with switchable electroosmotic flow

✍ Scribed by Smith, Joel Tim ;Rassi, Ziad El

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 607 KB
• Volume: 15
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240150903

✦ Synopsis

Abstract

A surface modification has been developed which yields fused silica capillaries with switchable electroosmotic flow (anodal/cathodal). The capillary surface is a composite material consisting of unreacted silanol groups, a layer of positively charged quaternary ammonium functions, and a hydrophilic layer of long polyether chains. Because of the presence of positively and negatively charged groups, the net charge of the capillary surface can be varied from positive to negative by changing the pH of the running electrolyte, thus enabling manipulation of the magnitude and direction of the electroosmotic flow. The long polyether chains were effective in shielding biomacromolecules from the charged inner surface of the capillary, thus minimizing electrostatic interaction of the solutes with both unreacted silanols and the quaternary ammonium groups which had been introduced. As a consequence, high separation efficiencies were achieved with proteins, nucleotides, and a series of acidic oligosaccharides.