Capillary supercritical fluid chromatography with flame photometric detection using a large bore column SFC pumping system

✍ Scribed by Koizumi, Hitoshi ;Suzuki, Yoshihito

• Book ID: 102894069
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 547 KB
• Volume: 14
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240140411

✦ Synopsis

Abstract

A commercially available instrument with an SFC pumping system suitable for wide bore columns (4.6 mm i.d.) has been modified for capillary supercritical fluid chromatography (CSFC) by incorporating a double‐stage flow splitter. The first flow splitter was installed in front of the sample injection valve in order to avoid a high solute split ratio. The second splitter was mounted in the column oven so that the injected sample (0.2 μL) would be split to the capillary column. In order to perform pressure programmed elution, a pressure regulating system equipped with a gradient programmer has been used. Flame photometric detection was optimized for the analysis of organosulfur compounds by CSFC. In this study, detection limits were found to be 6–14 ng and the experimentally determined exponent (n value) varied from 1.721 to 1.984 depending on the compounds tested. Sulfur‐ and phosphorus‐containing thermally labile pesticides can be chromatographed and selectively detected by using CSFC/FPD in either sulfur‐ or phosphorus mode, respectively.