Capillary electrophoresis with dual laser detection in separation of amplified fragment length polymorphism fragments

✍ Scribed by Justyna Stefanowicz-Hajduk; Natalia Filipowicz; Igor Kosinski; J. Renata Ochocka

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 338 KB
• Volume: 32
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200900297

✦ Synopsis

Abstract

We are presenting the application of CE technique with dual‐channel LIF detection for the simultaneous separation of DNA fragments labeled with two different fluorescence dyes. The optimal conditions of the analysis were determined for the separation of amplified fragment length polymorphism (AFLP) fragments labeled with 5′‐6‐carboxyfluorescein (6‐FAM) and the DNA size standard labeled with sulfoindocyanine succinimidyl ester (Cy‐5). CE equipped with both argon ion and diode lasers is a good alternative for sequencers and might be applied in analyses of PCR products generated by various fingerprinting methods.