Capillary electrophoresis with constant potential amperometric detection using a nickel microelectrode for detection of carbohydrates

✍ Scribed by Fermier, Adam M. ;Colón, Luis A.

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 352 KB
• Volume: 19
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240191104

✦ Synopsis

Abstract

Carbohydrates were separated by capillary electrophoresis (CE) and detected electrochemically using a nickel microelectrode which was operated at a constant applied potential (∼0.6 V vs. Ag/AgCI). A simple capillary electrode holder design facilitated alignment between the separation capillary and the working microelectrode without the use of micro‐positioning equipment. The separations were performed under alkaline conditions (pH > 11), matching the high pH requirements for amperometric detection at the nickel electrode. The analytical procedure developed showed detection limits for the carbohydrates studied in the micromolar range, showing a linear response in the range tested (micromolar to millimolar). The procedure was used to identify sugars in two real samples (i.e., urine and in a common beverage). The potential use of the system for the determination of amino acids was also demonstrated.