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Capillary Electrophoresis-SELEX Selection of Aptamers with Affinity for HIV-1 Reverse Transcriptase

✍ Scribed by Mosing, Renee K.; Mendonsa, Shaun D.; Bowser, Michael T.

Book ID
111696074
Publisher
American Chemical Society
Year
2005
Tongue
English
Weight
183 KB
Volume
77
Category
Article
ISSN
0003-2700

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Study of binding stoichiometries of the
Study of binding stoichiometries of the human immunodeficiency virus type 1 reverse transcriptase by capillary electrophoresis and laser-induced fluorescence polarization using aptamers as probes
✍ Hao Fu; Jeffreyβ€…W. Guthrie; X.β€…Chris Le πŸ“‚ Article πŸ“… 2006 πŸ› John Wiley and Sons 🌐 English βš– 179 KB

## Abstract Binding stoichiometries between four DNA aptamers (RT12, RT26, RTlt49, and ODN93) and the reverse transcriptase (RT) of the typeβ€…1 human immunodeficiency virus (HIV‐1) were studied using affinity CE (ACE) coupled with LIF polarization and fluorescence polarization (FP). The ACE/LIF stud