β Scribed by Philippe Schmitt-Kopplin, Philippe Schmitt-Kopplin
No coin nor oath required. For personal study only.
Capillary Electrophoresis: Methods and Protocols presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques. The volume is intended for beginners in the field and provides overviews of each technique and a starting point for the exploration of the defined literature on the different application topics. As a beginning text, each technique is explained in simple terms, assuming only minimal previous knowledge of the methods. A Notes section accompanies each chapter and addresses problems that may occur and how to overcome them.
In Part I, Analyte-Oriented, contributors present methods and protocols based on sample type and size for inorganic and organic small ions, anionic, cationic, and neutral pollutants and pharmaceuticals, sugars, amino acids, nucleotides and DNA, and synthetic copolymers up to microorganisms. In Part II, Methods-Oriented, authors present the more methodological descriptions of particular and new CE techniques including CZE, MEKC/MECC, CEC, affinity CE, coating and online concentration techniques, single-cell analysis, and multidimensional techniques. Comprehensive and timely, Capillary Electrophoresis: Methods and Protocols is ideal for researchers looking for a beginning text on capillary electrophoresis methods.
Cover......Page 1
Frontmatter......Page 2
I: Analyte-Oriented......Page 19
1 Determination of Small Ions With Capillary Electrophoresis and Contactless Conductivity Detection......Page 20
2 Metal Analysis With Capillary Zone Electrophoresis......Page 37
3 Measurement of Low-Molecular-Weight Carboxylic Acids in Ambient Air and Vehicle Emission by Capillary Electrophoresis......Page 59
4 Determination of Aliphatic Low-Molecular-Weight and Biogenic Amines by Capillary Zone Electrophoresis......Page 81
5 Capillary Electrophoretic Analysis of Organic Pollutants......Page 108
6 Capillary Electrophoresis With Laser-Induced Fluorescence - Environmental Applications......Page 134
7 Practical Considerations for the Analysis of Ionic and Neutral Organic Molecules With Capillary Electrophoresis/Mass Spectrometry......Page 150
8 Analysis of the Enantiomers of Chiral Pesticides and Other Pollutants in Environmental Samples by Capillary Electrophoresis......Page 172
9 Capillary Electrophoresis of Tropane Alkaloids and Glycoalkaloids Occurring in Solanaceae Plants......Page 186
10 Capillary Electrophoresis for Pharmaceutical Analysis......Page 219
11 Capillary Electrophoresis of Neutral Carbohydrates - Mono-, Oligosaccharides, and Glycosides......Page 260
12 Capillary Electrophoresis of Sugar Acids......Page 319
13 Use of Capillary Electrophoresis for Polysaccharide Studies and Applications......Page 368
14 Analysis of Oligonucleotides Using Capillary Zone Electrophoresis and Electrospray Mass Spectrometry......Page 412
15 Separation of DNA by Capillary Electrophoresis......Page 426
16 Capillary Electrophoresis of Oxidative DNA Damage......Page 441
17 Capillary Electrophoresis of Gene Mutation......Page 451
18 Biomedical Applications of Amino Acid Detection by Capillary Electrophoresis......Page 466
19 Separation of Peptides by Capillary Electrophoresis......Page 491
20 Analysis of Proteins by Capillary Electrophoresis......Page 515
21 Separation of Synthetic (Co)Polymers by Capillary Electrophoresis Techniques......Page 549
22 Capillary Electrophoresis Separation of Microorganisms......Page 576
II: Methods-Oriented......Page 598
23 A Semi-Empirical Approach for a Rapid Comprehensive Evaluation of the Electrophoretic Behaviors of Small Molecules in Free-Zone Electrophoresis......Page 599
24 The CE Way of Thinking - βAll is Relative!β......Page 616
25 Adsorbed Cationic Polymer Coatings for Enhanced Capillary Electrophoresis/Mass Spectrometry of Proteins......Page 635
26 On-Column Ligand/Receptor Derivatization Coupled to Affinity Capillary Electrophoresis......Page 651
27 On-Line Concentration of Environmental Pollutant Samples by Using Capillary Electrophoresis......Page 665
28 Free-Flow Electrophoresis System for Proteomics Applications......Page 706
29 Microemulsion Electrokinetic Chromatography......Page 720
30 Micellar Electrokinetic Chromatography of Aminoglycosides......Page 737
31 Capillary Electrochromatography and On-Line Concentration......Page 752
32 Analysis of Alkaloids in Single Plant Cells by Capillary Electrophoresis......Page 771
33 Multi-Dimensional Capillary Electrophoresis and Chromatography for Proteomic Analysis......Page 783
Index......Page 802
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