✦ LIBER ✦

Capillary electrophoresis Fourier transform ion cyclotron resonance mass spectrometry with sustained off-resonance irradiation for the characterization of protein and peptide mixtures

✍ Scribed by Steven A. Hofstadler; Jon H. Wahl; Ray Bakhtiar; Gordon A. Anderson; James E. Bruce; Richard D. Smith

Publisher: Elsevier Science
Year: 1994
Tongue: English
Weight: 604 KB
Volume: 5
Category: Article
ISSN: 1044-0305
DOI: 10.1016/1044-0305(94)87014-4

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✦ Synopsis

A new approach to protein and peptide analysis that involves the coupling of on-line capillary electrophoresispelectrospray ionization Fourier transform ion cyclotron resonance mass spectrometry with a variation of sustained off-resonance irradiation is described. With this technique, multiple irradiation frequencies are broadcast simultaneously, which yields fragmentation of species at different mass-to-charge ratio values from the same waveform. In conjunction with capillary electrophoresis, this technique can provide sequence information from small amounts of proteins or peptides i,n complex mixtures. Initial results obtained from a mixture of gramicidin S (1141 u)

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