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Capillary column gas-liquid chromatographic resolution of oxidized cholesterol derivatives

✍ Scribed by S.Won Park; P.B. Addis

Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
642 KB
Volume
149
Category
Article
ISSN
0003-2697

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✦ Synopsis

Fused-silica capillary columns were evaluated for the resolution of oxidized cholesterol derivatives. Thermal instability of diol derivatives, epimeric 7~ and 7fl-hydroxy, 4&hydroxy, and 25-hydroxycholesterol, was observed during gas chromatography. After derivatization as trimethylsilyl ethers the foregoing diols, cY-epoxide, cholestane-triol, 7-ketocholesterol, and cholesta-3,5-dien-7-one were completely resolved on a DB-1 column. Each oxidized sterol revealed excellent response linearity as the trimethylsilylated sterol, enabling reliable quantification. The identity of each derivatized sterol was confirmed by mass spectrometry.

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## Abstract Capillary columns of 0.3‐0.5 mm i.d. packed with 3‐ to 30‐μm silica‐based stationary phases for liquid chromatography were used for gas chromatographic separation of hydrocarbons. Column efficiencies were evaluated for various commercially available packing material. The best column eff