Capacity of B-lymphocytic lines of diverse tumor origin to produce and respond to B-cell growth factors: A progression model for B-cell lymphomagenesis
✍ Scribed by John Gordon; Pierre Åman; Anders Rosén; Ingemar Ernberg; Barbro Ehlin-Henriksson; George Klein
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- French
- Weight
- 624 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Abstract
Human cell lines established from cases of acute lymphoblastic leukemia, lymphosarcoma, Burkitt's lymphoma and multiple myeloma and representing stages of B‐lymphocyte development ranging from pre‐B through to plasma cells, were assessed for their ability to produce and respond to B‐cell growth factors (BCGF). All B‐cell lines studied were found to be constitutive producers of a growth activity which assisted the S‐phase entry of normal activated B‐cells and provided growth support for lymphoblastoid cells transformed by Epstein‐Barr virus. Furthermore, all lines responded by enhanced proliferation to supernatants from a BCGF‐producing T‐cell hybridoma. Not all lines, however, displayed autostimulation to their own supernatants and no tumor B‐cell line appeared totally dependent on soluble factors for its growth. Non‐tumorigenic B‐cell lines, by contrast, revealed a strict dependency on homologous growith factor for their continued proliferation in suspension culture. The findings support a progression model of lymphomagenesis based upon the utilization, production and, ultimately, emancipation from growth‐promoting soluble factors.