Capacitively coupled contactless conductivity detection as an alternative detection mode in CE for the analysis of kanamycin sulphate and its related substances
✍ Scribed by Mohamed N. El-Attug; Erwin Adams; Jos Hoogmartens; Ann Van Schepdael
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 186 KB
- Volume
- 34
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
A method was developed to determine simultaneously kanamycin, its related substances and sulphate in kanamycin sulphate using capacitively coupled contactless conductivity detection. Kanamycin is an aminoglycoside antibiotic that lacks a strong UV‐absorbing chromophore. Due to its physicochemical properties, CE in combination with capacitively coupled contactless conductivity detection was chosen. The separation method uses a BGE composed of 40 mM 2‐(N‐morpholino)ethanesulphonic acid monohydrate and 40 mM L‐histidine, pH 6.35. A 0.6 mM N‐cetyltrimethyl ammonium bromide (CTAB) solution was added as electroosmotic flow modifier in a concentration below the critical micellar concentration (CMC). Ammonium acetate 50 mg/L was used as internal standard. In total, 30 kV was applied in reverse polarity on a fused‐silica capillary (65/41 cm; 75 μm id). The optimized separation was obtained in less than 6 min with good linearity (R^2^=0.9999) for kanamycin. It shows a good precision expressed as RSD on the relative peak areas equal to 0.3 and 1.1% for intra‐day and inter‐day precision, respectively. The LOD and LOQ are 0.7 and 2.3 mg/L, respectively. Similarly, for sulphate, a good linearity (R^2^=0.9996) and precision (RSD 0.4 and 0.6% for intra‐day and inter‐day, respectively) were obtained.