Capacitative calcium influx in human epithelial breast cancer and non-tumorigenic cells occurs through Ca2+ entry pathways with different permeabilities to divalent cations
✍ Scribed by Carolina Baldi; Guillermo Vazquez; Ricardo Boland
- Publisher
- John Wiley and Sons
- Year
- 2003
- Tongue
- English
- Weight
- 153 KB
- Volume
- 88
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
Abstract
The operation of capacitative Ca^2+^ entry (CCE) in human breast cancer (SKBR3) and non‐tumorigenic (HBL100) cell lines was investigated as an alternative Ca^2+^ entry route in these cells. Ca^2+^ readdition after thapsigargin‐induced store depletion showed activation of CCE in both cell lines. SKBR3 cells exhibited retarded store depletion and CCE decay kinetics compared to the non‐tumorigenic HBL100 cells, suggesting alterations in Ca^2+^ homeostasis. CCE was also highly permeable to Mn^2+^ and to a lesser extent to Sr^2+^, but not to Ba^2+^. In HBL100 cells, CCE is contributed (30%) by a Ca^2+^/Mn^2+^ permeable route insensitive to low (1 μM) Gd^3+^ and a Ca^2+^/Sr^2+^/Mn^2+^ permeable non‐selective pathway (70%) sensitive to 1 μM Gd^3+^. In SKBR3 cells, the relative contribution to CCE of both routes was opposite to that in non‐tumorigenic cells. J. Cell. Biochem. 88: 1265–1272, 2003. © 2003 Wiley‐Liss, Inc.