Capacitative Ca2+ influx in the neural retina of chick embryo

✍ Scribed by Sakaki, Yoko ;Sugioka, Miho ;Fukuda, Yutaka ;Yamashita, Masayuki

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 245 KB
• Volume: 32
• Category: Article
• ISSN: 0022-3034
• DOI: 10.1002/(sici)1097-4695(199701)32:1<62::aid-neu6>3.0.co;2-c

✦ Synopsis

Depletion of intracellular Ca 2/ gradually until E13, when the Ca 2/ rise almost disapstores induces a capacitative Ca 2/ influx in non-neural peared. This developmental profile correlated with the cells. It has been unknown whether the capacitative Ca 2/ influx occurs in the cells of nervous systems. We decline in the mitotic activities of the retinal cells studfound the capacitative Ca 2/ influx in the neural retina ied by Prada et al. The fluorescence imaging with the of early embryonic chick with Fura-2 fluorescence

vertical slice of the E9 retina showed that the site at measurements. A Ca 2/ -free medium containing thapwhich the thapsigargin-induced Ca 2/ rise was largest sigargin (500 nM), an inhibitor of Ca 2/ -ATPase of was the most outer layer of the retina, where proliferintracellular Ca 2/ stores, was applied to the neural ating cells are located. This spatial distribution and retina of embryonic day 3 (E3) chick. A rise in intrathe above developmental profile may suggest that the cellular Ca 2/ concentration was evoked after the reincapacitative Ca 2/ influx occurs at the early period of troduction of extracellular Ca 2/ , and this Ca 2/ rise neurogenesis when the cells have mitotic activities. was suppressed by Zn 2/ (1 mM) and Ni 2/ (5 mM).