Cancer Cell Signaling: Methods and Protocols

✍ Scribed by Erik S. Knudsen, Steven P. Angus (auth.), David M. Terrian (eds.)

• Publisher: Humana Press
• Year: 2003
• Tongue: English
• Leaves: 329
• Series: Methods in Molecular Biology™ 218
• Edition: 1
• Category: Library

✦ Synopsis

The rapidly evolving nature of cell signaling research in cancer has necessitated a continuous updating of the methods used in its study, most recently to take into account the analysis of multiple members within a gene family. Cancer Cell Signaling: Methods and Protocols brings together all the major methods of cell research that are scientifically grounded within the cancer biology field. Presented in step-by-step detail to ensure successful results, the methods include proven techniques for the investigation of apoptosis and cell death, complementary protocols for manipulating and/or monitoring oncogenic signals in cancer cells, and techniques for studying protein-protein interactions. Finally, there is a detailed protocol for capturing pure samples of malignant cells from frozen tissue specimens and two alternative techniques for analyzing their genomic DNA. Each method is described by a hands-on master of the technique and includes invaluable notes on troubleshooting and pitfalls to avoid.
Cutting-edge and highly practical, Cancer Cell Signaling: Methods and Protocols provides today's cancer researchers with new, but well-proven, strategies and procedures for investigating how the signaling cascades essential to normal cell life are corrupted, as well as for developing new molecular tools for cancer therapy.

✦ Table of Contents

Front Matter....Pages i-xiii
Front Matter....Pages 1-1
Front Matter....Pages 3-15
Front Matter....Pages 17-33
Front Matter....Pages 35-46
Back Matter....Pages 47-57
....Pages 59-69

✦ Subjects

Oncology