Can you trust your cryostat? Reproducibility of cryostat section thickness
✍ Scribed by Philip C. De Witt Hamer; Fonnet E. Bleeker; Aeilko H. Zwinderman; Cornelis J.F. Van Noorden
- Book ID
- 102333180
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 96 KB
- Volume
- 69
- Category
- Article
- ISSN
- 1059-910X
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✦ Synopsis
Abstract
Reproducibility of cryostat section thickness is required for valid quantitative microscopy. This is generally pursued by motorized sectioning using a low but constant speed. The purpose of our study was to compare variation in section thickness between motorized and manual cryostat sectioning. Serial sections were cut from a frozen block of homogenized tissue on different days. Lactate dehydrogenase activity was histochemically detected and calibrated absorbance measurements were taken. The coefficients of variation of measurements was 9.7% for motorized sectioning and 3.3% for manual sectioning. In conclusion, section thickness is similarly reproducible after manual sectioning compared with motorized sectioning, if not better. Microsc. Res. Tech., 2006. © 2006 Wiley‐Liss, Inc.