Can sister chromatid intercrossings be considered as prelesions?

✍ Scribed by Carme Fuster; Rosa Mirò; Cristina Templado; Leonardo Barrios; Josep Egozcue

• Book ID: 104701850
• Publisher: Springer
• Year: 1988
• Tongue: English
• Weight: 297 KB
• Volume: 79
• Category: Article
• ISSN: 0340-6717
• DOI: 10.1007/bf00280561

✦ Synopsis

In a study of the expression of folate-sensitive fragile sites in five normal individuals using RPMI medium containing methotrexate (MTX), we observed a high frequency of "sister chromatid intercrossings" (SCI) that is, the intersection of sister chromatids. The location of SCIs corresponded to fragile sites in 54.2% of the cases. Of the SCIs observed in each individual, 43%-53% were located at the same bands as their expressed fragile sites. Furthermore when RPMI + MTX medium was used instead of F-10 medium, the incidence of SCIs increased tenfold. We suggest that SCIs could indicate the existence of a pre-lesion.