Can keratin 8 and 17 immunohistochemistry be of diagnostic value in cervical cytology?
✍ Scribed by Martens, Jolise ;Baars, Janny ;Smedts, Frank ;Holterheus, Marjon ;Kok, Marie-Jose ;Vooijs, Peter ;Ramaekers, Frans
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 251 KB
- Volume
- 87
- Category
- Article
- ISSN
- 0008-543X
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✦ Synopsis
BACKGROUND.
Based on results from evaluation of tissue sections from premalignant lesions of the uterine cervix, the authors examined the hypothesis that immunostaining of Papanicolaou-stained cytologic smears with monoclonal antibodies to keratins 8 and 17 allows detection of cervical intraepithelial neoplasia (CIN) with progressive potential. They also investigated whether detection of these two keratin subtypes could be of help in the analysis of normal and/or poor quality cytology smears.
METHODS.
Sixty-one Papanicolaou-stained smears, representing 25 normal smears, 8 CIN 1, 7 CIN 2, 18 CIN 3, and 3 cervical carcinomas, were stained with CAM 5.2 and E3, which are capable of detecting keratin 8 and 17, respectively. The percentages of immunoreactive normal, metaplastic, dysplastic, and malignant epithelial cells were determined.