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Calorimetric dissection of thermal unfolding of OspA, a predominantly β-sheet protein containing a single-layer β-sheet

✍ Scribed by Tomoko Nakagawa; Hirotaka Shimizu; Karl Link; Akiko Koide; Shohei Koide; Atsuo Tamura


Book ID
117143057
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
660 KB
Volume
323
Category
Article
ISSN
0022-2836

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The unfolding dynamics of cellular retinoic acid-binding protein I (CRABP I), an 18 kDa predominantly b-sheet protein, were studied by monitoring the hydrogen-deuterium (H-D) exchange reaction under various solution conditions. A bimodal charge state distribution was observed when a denaturing agent