Calibration Point for Liquid Secondary Ion MassSpectrometry Tandem Mass Spectra Measuredwith an EBqQ Hybrid Mass Spectrometer
✍ Scribed by Lemire, Sharon W.; Busch, Kenneth L.
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 833 KB
- Volume
- 31
- Category
- Article
- ISSN
- 1076-5174
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✦ Synopsis
A protocol for establishing standard instrument conditions for the measurement of product ion tandem mass spectra from parent ions generated by liquid secondary ion mass spectrometry (LSIMS) is presented. The ion at m/z 319 (C') from 2-butyltriphenylphosphonium bromide is selected as the parent ion and then is subjected to collision-induced dissociation. The relative abundances of product ions at m/z 263 and 183 are monitored as a function of collision energy while keeping the target gas pressure constant. The collision energy at which these two most abundant product ions are equal is defined as the calibration point at that collision gas pressure. LSIMS tandem mass spectra acquired at the calibration point show good reproducibility for several model compounds. Spectra obtained at the calibration point at two different collision gas pressures, representing multiple and single collision conditions, also show a high degree of similarity. Use of this protocol may aid in the establishment of a tandem mass spectral library.