Calibration of Optical Tweezers for In Vivo Force Measurements: How do Different Approaches Compare?

New calibration method for position dete

New calibration method for position detector for simultaneous measurements of force constants and local viscosity in optical tweezers

✍ A. Buosciolo; G. Pesce; A. Sasso 📂 Article 📅 2004 🏛 Elsevier Science 🌐 English ⚖ 335 KB

We present a new method to calibrate a quadrant photodiode used as position detector to monitor latex beads trapped in optical tweezers. The method combines the dragging StokeÕs force with the thermal noise analysis (power spectral density (PSD)) of the Brownian motion of the trapped bead. Position