Calibration of a wide-field frequency-domain fluorescence lifetime microscopy system using light emitting diodes as light sources

Characterization of biological materials

Characterization of biological materials by frequency-domain fluorescence lifetime measurements using ultraviolet light-emitting diodes

✍ A. Žukauskas; P. Vitta; N. Kurilčik; S. Juršėnas; E. Bakienė 📂 Article 📅 2008 🏛 Elsevier Science 🌐 English ⚖ 246 KB

Recently developed deep-ultraviolet light-emitting diodes (UV LEDs) emit at wavelengths short enough to excitate fluorescence in most biological autofluorophores. We demonstrate the possibility of harmonical modulation of the output of group-III-nitride based UV LEDs ranging from 255 to 375 nm at fr