An automated method for measurement of proteinase activities using fluorogenic substrates is described. Enzyme assays were performed' in polystyrene microtitration trays as normally used for the enzyme-linked immunosorbent assay technique. The reaction products were measured using an inverted fluore
Calibration of a surgical microscope with automated zoom lenses using an active optical tracker
✍ Scribed by Jaime García; Ramesh Thoranaghatte; Gaetan Marti; Guoyan Zheng; Marco Caversaccio; Miguel A. González Ballester
- Book ID
- 104583195
- Publisher
- Wiley (Robotic Publications)
- Year
- 2008
- Tongue
- English
- Weight
- 678 KB
- Volume
- 4
- Category
- Article
- ISSN
- 1478-5951
- DOI
- 10.1002/rcs.180
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Background
In this paper, we present a new method for the calibration of a microscope and its registration using an active optical tracker.
Methods
Practically, both operations are done simultaneously by moving an active optical marker within the field of view of the two devices. The IR LEDs composing the marker are first segmented from the microscope images. By knowing their corresponding three‐dimensional (3D) position in the optical tracker reference system, it is possible to find the transformation matrix between the referential of the two devices. Registration and calibration parameters can be extracted directly from that transformation. In addition, since the zoom and focus can be modified by the surgeon during the operation, we propose a spline based method to update the camera model to the new setup.
Results
The proposed technique is currently being used in an augmented reality system for image‐guided surgery in the fields of ear, nose and throat (ENT) and craniomaxillofacial surgeries.
Conclusions
The results have proved to be accurate and the technique is a fast, dynamic and reliable way to calibrate and register the two devices in an OR environment. Copyright © 2008 John Wiley & Sons, Ltd.
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