Calcyclin binding protein promotes DNA synthesis and differentiation in rat neonatal cardiomyocytes
✍ Scribed by Ka-Wing Au; Cecy Y.C. Kou; Anthony Y.H. Woo; Stephen S.C. Chim; Kwok-Pui Fung; Christopher H.K. Cheng; Mary M.Y. Waye; Stephen K.W. Tsui
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 392 KB
- Volume
- 98
- Category
- Article
- ISSN
- 0730-2312
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
During cardiac muscle development, most cardiomyocytes permanently withdraw from the cell cycle. Previously, by suppressive subtractive hybridization, we identified calcyclin‐binding protein/Siah‐interacting protein (CacyBP/SIP) as one of the candidates being upregulated in the hyperplastic to hypertrophic switch, suggesting an important role of CacyBP/SIP in cardiac development. To show the importance of CacyBP/SIP during myoblast differentiation, we report here that CacyBP/SIP is developmentally regulated in postnatal rat hearts. The overexpression of CacyBP/SIP promotes the differentiation and DNA synthesis of H9C2 cells and primary rat cardiomyocytes, as well as downregulates the expression of β‐catenin. Besides, CacyBP/SIP promotes the formation of myotubes and multinucleation upon differentiation. To investigate the cardioprotective role of CacyBP/SIP in cardiomyocytes, a hypoxia/reoxygenation model was employed. We found that CacyBP/SIP was upregulated during myocardial infarction (MI) and hypoxia/reoxygenation. As a conclusion, CacyBP/SIP may play a role in cardiomyogenic differentiation and possibly protection of cardiomyocytes during hypoxia/reoxygenation injury. J. Cell. Biochem. 98: 555–566, 2006. © 2006 Wiley‐Liss, Inc.