Calculating extinction coefficients for enzymatically produced o-quinones

✍ Scribed by J.Herbert Waite

• Publisher: Elsevier Science
• Year: 1976
• Tongue: English
• Weight: 425 KB
• Volume: 75
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(76)90072-5

✦ Synopsis

Molar extinction coefficients of o-quinones produced from o-diphenols by the action of o-diphenok0, oxidoreductase were calculated by least-squares linear regression at several wavelengths in the pH range 5.8-7.8. The accuracy of each extinction coefficient is verified by the standard errors of the regression coefficient and the estimate, and by 95% confidence limits. The seven o-diphenols used in the present study were pyrocatechol, 4-methylcatechol, Ctertiarybutylcatechol, 3,4-L-dihydroxyphenylalanine, 3,4-dihydroxyphenylethylamine. 3,4-dihydroxyphenylacetic acid, and 3.4-dihydroxyhydrocinnamic acid.