Calcium transport and Ca++-ATPase activity in spermatozoal plasma membrane vesicles of nifedipine-administered guinea pigs
β Scribed by R. Juneja; I. Gupta; A. Wali; R.N. Chakravarti; S. Majumdar
- Book ID
- 115844804
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 528 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0010-7824
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## Abstract The phenothiazine, trifluoperazine, and the mitogenic lectins, phytohemagglutinin (PHA) and Concanavalin A (Con A), were tested for their effects on human lymphocyte plasma membrane Caβactivated MgβATPase and ATPβdependent calcium uptake. Trifluoperazine completely inhibited Caβuptake w
Partially purified plasma membrane fractions were prepared from guinea-pig pancreatic acini. These membrane preparations were found to contain an ATP-dependent Ca(2+)-transporter as well as a heterogenous ATP-hydrolytic activity. The Ca(2+)-transporter showed high affinity for Ca2+ (KCa2+ = 0.04 +/-